Primer

Part:BBa_K3658005:Design

Designed by: Marko Susic   Group: iGEM20_NYU_Abu_Dhabi   (2020-10-24)


Batrachochytrium salamandrivorans (Bsal) ITS region RPA reverse primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primers generated were annotated in Benchling and optimal primers were selected based on location, GC content, fitness with PCR amplicon, and quality of available gRNAs within the amplified region.


Source

We found the Bsal ITS region sequence in NCBI and used primer-BLAST and PrimedRPA software to design the primer.

References